Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice

Claudia Torero Gutierrez; Charis Loizides; Iosif Hafez; Anders Brostrøm; Henrik Wolff; Józef Szarek; Trine Berthing; Alicja Mortensen; Keld Alstrup Jensen; Martin Roursgaard; Anne Thoustrup Saber; Peter Møller; George Biskos; Ulla Vogel

doi:10.1186/s12989-023-00514-0

Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice

Claudia Torero Gutierrez, Charis Loizides, Iosif Hafez, Anders Brostrøm, Henrik Wolff, Józef Szarek, Trine Berthing, Alicja Mortensen, Keld Alstrup Jensen, Martin Roursgaard, Anne Thoustrup Saber, Peter Møller, George Biskos, Ulla Vogel

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al2O3, SnO2 and TiO2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues.

Originalsprog	Engelsk
Tidsskrift	Particle and Fibre Toxicology
Vol/bind	20
Udgave nummer	1
Sider (fra-til)	4
ISSN	1743-8977
DOI	https://doi.org/10.1186/s12989-023-00514-0
Status	Udgivet - 17 jan. 2023

Emneord

Nano
Toksikologi
Partikler
Hjertekarsygdomme
Kemisk arbejdsmiljø

Adgang til dokumentet

10.1186/s12989-023-00514-0

FFIKA: Fornyet fokus på forskning i kemisk arbejdsmiljø
Vogel, U. B. (Projektleder), Jensen, K. A. (Projektdeltager), Jensen, A. C. Ø. (Projektdeltager), Berthing, T. (Projektdeltager), Andersen, M. H. G. (Projektdeltager), Hougaard, K. S. (Projektdeltager), Clausen, P. A. (Projektdeltager), Frederiksen, M. (Projektdeltager), Mortensen, A. (Projektdeltager), Saber, A. T. (Projektdeltager), Jacobsen, N. R. (Projektdeltager), Akhtar, Y. (Projektdeltager), Asp, A.-K. (Projektdeltager), Abildtrup, A. (Projektdeltager), Tegner, U. (Projektdeltager), Terrida, E. B. (Projektdeltager), Guldbrandsen, M. (Projektdeltager), Kembouche, Y. (Projektdeltager), Kofoed-Sørensen, V. (Projektdeltager), Nielsen, S. H. (Projektdeltager), Sahlgren, N. M. (Projektdeltager), Fonseca, A. S. (Projektdeltager), Danielsen, P. H. (Projektdeltager), Sørli, J. B. (Projektdeltager), Hadrup, N. (Projektdeltager), Ardenkjær-Skinnerup, J. (Projektdeltager), Nøjgaard, J. N. K. (Projektdeltager), Jørgensen, A. K. (Projektdeltager), Gutierrez, C. A. T. (Projektdeltager) & Brostrøm, A. (Projektdeltager)
01/01/2020 → 31/12/2026
Projekter: Projekt › Forskning

Citationsformater

Gutierrez, C. T., Loizides, C., Hafez, I., Brostrøm, A., Wolff, H., Szarek, J., Berthing, T., Mortensen, A., Jensen, K. A., Roursgaard, M., Saber, A. T., Møller, P., Biskos, G., & Vogel, U. (2023). Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice. Particle and Fibre Toxicology, 20(1), 4. https://doi.org/10.1186/s12989-023-00514-0

@article{db5b02d5a5bb454d8534afaaa14078f6,

title = "Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice",

abstract = "BACKGROUND: Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al 2O 3, SnO 2 and TiO 2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues. RESULTS: All nanomaterials induced pulmonary inflammation after 1 day, and exposure to ZnO, CuO, SnO 2, TiO 2 and Printex 90 increased Saa3 mRNA levels in lungs and Saa1 mRNA levels in liver. Additionally, CuO, SnO 2, TiO 2 and Printex 90 increased plasma levels of SAA3 and SAA1/2. Acute phase response was predicted by deposited surface area for insoluble metal oxides, 1 and 28 days post-exposure. CONCLUSION: Soluble and insoluble metal oxides induced dose-dependent APR with different time dependency. Neutrophil influx, Saa3 mRNA levels in lung tissue and plasma SAA3 levels correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.",

keywords = "Nano, Toksikologi, Partikler, Hjertekarsygdomme, Kemisk arbejdsmilj{\o}, Mice, Animals, Acute-Phase Reaction/chemically induced, Zinc Oxide/toxicity, Lung/metabolism, Nanostructures/toxicity, RNA, Messenger/genetics",

author = "Gutierrez, {Claudia Torero} and Charis Loizides and Iosif Hafez and Anders Brostr{\o}m and Henrik Wolff and J{\'o}zef Szarek and Trine Berthing and Alicja Mortensen and Jensen, {Keld Alstrup} and Martin Roursgaard and Saber, {Anne Thoustrup} and Peter M{\o}ller and George Biskos and Ulla Vogel",

year = "2023",

month = jan,

day = "17",

doi = "10.1186/s12989-023-00514-0",

language = "English",

volume = "20",

pages = "4",

journal = "Particle and Fibre Toxicology",

issn = "1743-8977",

publisher = "BioMed Central",

number = "1",

}

Gutierrez, CT, Loizides, C, Hafez, I, Brostrøm, A, Wolff, H, Szarek, J, Berthing, T , Mortensen, A , Jensen, KA, Roursgaard, M, Saber, AT, Møller, P, Biskos, G & Vogel, U 2023, 'Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice', Particle and Fibre Toxicology, bind 20, nr. 1, s. 4. https://doi.org/10.1186/s12989-023-00514-0

TY - JOUR

T1 - Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice

AU - Gutierrez, Claudia Torero

AU - Loizides, Charis

AU - Hafez, Iosif

AU - Brostrøm, Anders

AU - Wolff, Henrik

AU - Szarek, Józef

AU - Berthing, Trine

AU - Mortensen, Alicja

AU - Jensen, Keld Alstrup

AU - Roursgaard, Martin

AU - Saber, Anne Thoustrup

AU - Møller, Peter

AU - Biskos, George

AU - Vogel, Ulla

PY - 2023/1/17

Y1 - 2023/1/17

N2 - BACKGROUND: Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al 2O 3, SnO 2 and TiO 2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues. RESULTS: All nanomaterials induced pulmonary inflammation after 1 day, and exposure to ZnO, CuO, SnO 2, TiO 2 and Printex 90 increased Saa3 mRNA levels in lungs and Saa1 mRNA levels in liver. Additionally, CuO, SnO 2, TiO 2 and Printex 90 increased plasma levels of SAA3 and SAA1/2. Acute phase response was predicted by deposited surface area for insoluble metal oxides, 1 and 28 days post-exposure. CONCLUSION: Soluble and insoluble metal oxides induced dose-dependent APR with different time dependency. Neutrophil influx, Saa3 mRNA levels in lung tissue and plasma SAA3 levels correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.

AB - BACKGROUND: Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al 2O 3, SnO 2 and TiO 2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues. RESULTS: All nanomaterials induced pulmonary inflammation after 1 day, and exposure to ZnO, CuO, SnO 2, TiO 2 and Printex 90 increased Saa3 mRNA levels in lungs and Saa1 mRNA levels in liver. Additionally, CuO, SnO 2, TiO 2 and Printex 90 increased plasma levels of SAA3 and SAA1/2. Acute phase response was predicted by deposited surface area for insoluble metal oxides, 1 and 28 days post-exposure. CONCLUSION: Soluble and insoluble metal oxides induced dose-dependent APR with different time dependency. Neutrophil influx, Saa3 mRNA levels in lung tissue and plasma SAA3 levels correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.

KW - Nano

KW - Toksikologi

KW - Partikler

KW - Hjertekarsygdomme

KW - Kemisk arbejdsmiljø

KW - Mice

KW - Animals

KW - Acute-Phase Reaction/chemically induced

KW - Zinc Oxide/toxicity

KW - Lung/metabolism

KW - Nanostructures/toxicity

KW - RNA, Messenger/genetics

U2 - 10.1186/s12989-023-00514-0

DO - 10.1186/s12989-023-00514-0

M3 - Journal article

C2 - 36650530

SN - 1743-8977

VL - 20

SP - 4

JO - Particle and Fibre Toxicology

JF - Particle and Fibre Toxicology

IS - 1

ER -

Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice

Abstract

Emneord

Adgang til dokumentet

Fingeraftryk

Projekter

FFIKA: Fornyet fokus på forskning i kemisk arbejdsmiljø

Citationsformater