Projekter pr. år
Abstract
Antifungal resistance has emerged as a significant health concern with increasing reports of resistant variants in previously susceptible species. At present, little is known about occupational exposure to antifungal-resistant fungi. This study aimed to investigate Danish workers' occupational exposure to airborne fungi resistant to first-line treatment drugs. A retrospective study was performed on a unique collection of personal exposure samples gathered over a twenty-year period from Danish working environments, in sectors including agriculture, animal handling, waste management, and healthcare. A total of 669 samples were cultivated at 37 °C and fungal colonies were identified using MALDI-TOF MS. Subsequently, identification was confirmed by amplicon sequencing the genes of calmodulin and beta-tubulin to unveil potential cryptic species. Infectious fungi (495 isolates from 23 species) were tested for resistance against Itraconazole, Voriconazole, Posaconazole, and Amphotericin B. Working environments were highly variable in the overall fungal exposure, and showed vastly different species compositions. Resistance was found in 30 isolates of the species Aspergillus fumigatus (4 of 251 isolates), A. nidulans (2 of 13), A. niger complex (19 of 131), A. versicolor (3 of 18), and A. lentulus (2 of 2). Sequence analysis revealed several cryptic species within the A. niger complex including A. tubingensis, A. luchuensis, and A. phoenicis. Among the resistant A. fumigatus isolates, two contained the well-described TR34/L98H mutation in the cyp51A gene and promoter region, while the remainder harbored silent mutations. The results indicate that the working environment significantly contributes to exposure to resistant fungi, with particularly biofuel plant workers experiencing high exposure. Differences in the prevalence of resistance across working environments may be linked to the underlying species composition.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Science of the Total Environment |
Vol/bind | 923 |
Sider (fra-til) | 171189 |
ISSN | 0048-9697 |
DOI | |
Status | Udgivet - 1 maj 2024 |
Emneord
- mikrobiologi
- fungicidresistens
- antibiotikaresistens
- arbejdsrelateret eksponering
- biobrændsel
- gartneri
- dueavl
- SOSU
- svinebrug
- bioaffald
- rensningsanlæg
Fingeraftryk
Dyk ned i forskningsemnerne om 'Exposure to resistant fungi across working environments and time'. Sammen danner de et unikt fingeraftryk.Projekter
- 1 Afsluttet
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Eksponering for resistente svampe i arbejdsmiljøet
Madsen, A. M. (Projektleder), Tranchant, E. E. (Projektdeltager), Frederiksen, M. W. (Projektdeltager), Rasmussen, P. U. (Projektdeltager) & Kofoed, V. C. (Projektdeltager)
01/07/2021 → 31/12/2023
Projekter: Projekt › Forskning
Publikation
- 1 Tidsskriftartikel
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Effect of anaerobic digestion on pathogens and antimicrobial resistance in the sewage sludge
Franchitti, E., Pedullà, M., Madsen, A. M. & Traversi, D., sep. 2024, I: Environ Int. 191, s. 108998Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Åben adgang
Aktiviteter
- 2 Foredrag og mundtlige bidrag
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Præsentation af Fungicid projekt til følgegruppemøde om grøn omstilling
Kofoed, V. C. (Foredragsholder)
15 aug. 2023Aktivitet: Tale eller præsentation › Foredrag og mundtlige bidrag
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Præsentation af Fungicid projekt i Miljøstyrelsen
Kofoed, V. C. (Foredragsholder)
27 mar. 2023Aktivitet: Tale eller præsentation › Foredrag og mundtlige bidrag