Projekter pr. år
Abstract
Polycyclic aromatic compounds (PAHs) are formed during incomplete combustion and firefighters are inadvertently at risk of being exposed to these and other hazardous compounds. Exposure to PAHs is often estimated by measuring their hydroxylated metabolites (OH-PAH) in urine. Here, an online-SPE LC-MS-MS method was set up for eight OH-PAHs thus increasing sample throughput and minimizing manual handling. The method was validated over a 5-month period and showed good reproducibility with intra- and inter-day variation of 2.4-8.1% and 1.6-6.5%, respectively, of low-level samples and accuracy (91.6-104.8%) for a standard reference material. The method was applied to urine samples from conscripts training to become firefighters to determine the optimal sampling time for this training activity before a large intervention study. In total, six conscripts sampled urine 6-8 times over a 40-hr period during a 3-day training course. All eight metabolites were detected in ≥ 97% of the samples and showed peak excretion 4-6 hrs after the training corresponding to 8-10 hrs after first exposure. Samples taken the morning after the exercise contained low levels of most metabolites. Consequently, 4-6 hrs post exposure is recommended as the optimal sampling time for quantification of PAH exposure and monitoring of potential differences in exposure.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Toxicology Letters |
Vol/bind | 400 |
Sider (fra-til) | 9-15 |
Antal sider | 7 |
ISSN | 0378-4274 |
DOI | |
Status | Udgivet - okt. 2024 |
Emneord
- Brandmænd
- urin
- metamolitter
- biomonitering
- metoder
Fingeraftryk
Dyk ned i forskningsemnerne om 'Online SPE-LC-MS-MS method for eight hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine and determination of optimal sampling time after firefighter training'. Sammen danner de et unikt fingeraftryk.Projekter
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