Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles

Anda R Gliga; Sarah McCarrick; Vilhelm Malmborg; Pekka Kohonen; Anastasiia Snigireva; Brandon Mills; Pernille Høgh Danielsen; Lena Palmberg; Karin Broberg; Joakim Pagels; Ulla Vogel

doi:10.1016/j.etap.2025.104918

Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles

Anda R Gliga
, Sarah McCarrick
, Vilhelm Malmborg
, Pekka Kohonen
, Anastasiia Snigireva
, Brandon Mills
, Pernille Høgh Danielsen
, Lena Palmberg
, Karin Broberg
, Joakim Pagels
, Ulla Vogel

Karolinska Institutet
Lund University

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.

Originalsprog	Engelsk
Artikelnummer	104918
Tidsskrift	Environmental Toxicology and Pharmacology
Vol/bind	122
ISSN	1382-6689
DOI	https://doi.org/10.1016/j.etap.2025.104918
Status	Udgivet - mar. 2026

Emneord

Diesel
Partikler
Udstødning

Adgang til dokumentet

10.1016/j.etap.2025.104918

1 Igangværende

FFIKA: Fornyet fokus på forskning i kemisk arbejdsmiljø
Vogel, U. B. (Projektleder), Jensen, K. A. (Projektdeltager), Jensen, A. C. Ø. (Projektdeltager), Berthing, T. (Projektdeltager), Andersen, M. H. G. (Projektdeltager), Hougaard, K. S. (Projektdeltager), Clausen, P. A. (Projektdeltager), Frederiksen, M. (Projektdeltager), Mortensen, A. (Projektdeltager), Saber, A. T. (Projektdeltager), Jacobsen, N. R. (Projektdeltager), Akhtar, Y. (Projektdeltager), Asp, A.-K. (Projektdeltager), Abildtrup, A. (Projektdeltager), Tegner, U. (Projektdeltager), Terrida, E. B. (Projektdeltager), Guldbrandsen, M. (Projektdeltager), Kembouche, Y. (Projektdeltager), Kofoed-Sørensen, V. (Projektdeltager), Nielsen, S. H. (Projektdeltager), Sahlgren, N. M. (Projektdeltager), Fonseca, A. S. (Projektdeltager), Danielsen, P. H. (Projektdeltager), Sørli, J. B. (Projektdeltager), Hadrup, N. (Projektdeltager), Ardenkjær-Skinnerup, J. (Projektdeltager), Nøjgaard, J. N. K. (Projektdeltager), Jørgensen, A. K. (Projektdeltager), Gutierrez, C. A. T. (Projektdeltager) & Brostrøm, A. (Projektdeltager)
01/01/2020 → 31/12/2026
Projekter: Projekt › Forskning

1 Tidsskriftartikel

Pulmonary exposure to renewable diesel exhaust particles alters protein expression and toxicity profiles in bronchoalveolar lavage fluid and plasma of mice
McCarrick, S., Malmborg, V., Gren, L., Danielsen, P. H., Tunér, M., Palmberg, L., Broberg, K., Pagels, J., Vogel, U. & Gliga, A. R., feb. 2025, I: Archives of Toxicology. 99, 2, s. 797-814 18 s.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Åben adgang

Citationsformater

Gliga, A. R., McCarrick, S., Malmborg, V., Kohonen, P., Snigireva, A., Mills, B., Danielsen, P. H., Palmberg, L., Broberg, K., Pagels, J., & Vogel, U. (2026). Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles. Environmental Toxicology and Pharmacology, 122, Artikel 104918. https://doi.org/10.1016/j.etap.2025.104918

@article{8a8dc8dd078a4e7f818d10f79a290754,

title = "Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles",

abstract = "Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.",

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author = "Gliga, \{Anda R\} and Sarah McCarrick and Vilhelm Malmborg and Pekka Kohonen and Anastasiia Snigireva and Brandon Mills and Danielsen, \{Pernille H{\o}gh\} and Lena Palmberg and Karin Broberg and Joakim Pagels and Ulla Vogel",

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year = "2026",

month = mar,

doi = "10.1016/j.etap.2025.104918",

language = "English",

volume = "122",

journal = "Environmental Toxicology and Pharmacology",

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Gliga, AR, McCarrick, S, Malmborg, V, Kohonen, P, Snigireva, A, Mills, B, Danielsen, PH, Palmberg, L, Broberg, K, Pagels, J & Vogel, U 2026, 'Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles', Environmental Toxicology and Pharmacology, bind 122, 104918. https://doi.org/10.1016/j.etap.2025.104918

TY - JOUR

T1 - Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles

AU - Gliga, Anda R

AU - McCarrick, Sarah

AU - Malmborg, Vilhelm

AU - Kohonen, Pekka

AU - Snigireva, Anastasiia

AU - Mills, Brandon

AU - Danielsen, Pernille Høgh

AU - Palmberg, Lena

AU - Broberg, Karin

AU - Pagels, Joakim

AU - Vogel, Ulla

PY - 2026/3

Y1 - 2026/3

N2 - Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.

AB - Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.

KW - Diesel

KW - Partikler

KW - Udstødning

KW - omics

KW - Air Pollutants/toxicity

KW - Animals

KW - Mice, Inbred C57BL

KW - Vehicle Emissions/toxicity

KW - Transcriptome/drug effects

KW - Female

KW - Lung/drug effects

KW - Particulate Matter/toxicity

KW - Mice

KW - Gasoline/toxicity

U2 - 10.1016/j.etap.2025.104918

DO - 10.1016/j.etap.2025.104918

M3 - Journal article

C2 - 41456772

SN - 1382-6689

VL - 122

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

M1 - 104918

ER -

Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles

Abstract

Emneord

Adgang til dokumentet

Fingeraftryk

Projekter

FFIKA: Fornyet fokus på forskning i kemisk arbejdsmiljø

Publikation

Pulmonary exposure to renewable diesel exhaust particles alters protein expression and toxicity profiles in bronchoalveolar lavage fluid and plasma of mice

Citationsformater